KARAKTERISASI AKSESI DURIAN LOKAL KOLEKSI HORTIMART AGRO CENTRE JAWA TENGAH MENGGUNAKAN PENANDA MOLEKULER INTER SIMPLE SEQUENCE REPEATS (ISSR)

Ahmad Solikin, Amin Retnoningsih, Enni S. Rahayu

Abstract


Ahmad Solikin, Amin Retnoningsih & Enni S. Rahayu. 2017. Characterization of Local Durian Accession Collected Hortimart Agro Centre, Central Java Using Molecular Markers Inter Simple Sequence Repeats (ISSR). Floribunda 5(7): 267–276. — The lack of morphological markers encourage the use of other markers that are considered more accurate, i.e molecular markers. The purpose of this study is to analyze the genetic diversity as well as determine the identity of the local durian accession collection of Hortimart Agro Centre, Central Java. Leaves of 41 durian accessions were isolated using CTAB method. DNA amplification was conducted with four primers of ISSR PKBT and the results were separated by agarose gel. Data were analyzed using NTSYSpc program version 2: 02. The results showed that DNA amplification using four primers of ISSR BKPT yielded 66 polymorphic bands. The results of the analysis showed that the genetic diversity of local durians of Hortimart collection is high because 100% of polymorphic DNA bands with variations of size 250–1500 bp. No local durian accession was found to have the same DNA100% profile, although four pairs of accessions were known to have a 96% high similarity. Five accessions, namely Petruk 2, Ontoseno, Semar, Arjuno, and Gondomono have specific DNA bands. The only accession of durian apart from the other group is Gondomono because it has only one band on ISSR PKBT 8 with size 750 bp which is not owned by other accession. However, dendrograms showed that no identities that are 100% equal so that each
DNA band profile can be the identity of each durian accession.

Keywords: Durio zibethinus, molecular marker, genetic diversity.

Ahmad Solikin, Amin Retnoningsih & Enni S. Rahayu. 2017. Karakterisasi Aksesi Durian Lokal Koleksi Hortimart Agro Centre Jawa Tengah Menggunakan Penanda Molekuler Inter Simple Sequence Repeats (ISSR). Floribunda 5(7): 267–276. — Keterbatasan ciri morfologi mendorong penggunaan penanda lain yang dipandang lebih akurat, yaitu penanda molekuler. Tujuan penelitian ini adalah menganalisis keanekaragaman genetika sekaligus menentukan identitas aksesi durian lokal koleksi Hortimart Agro Centre, Bawen, Jawa Tengah. Daun 41 aksesi durian diisolasi menggunakan metode CTAB. Amplifikasi DNA dilakukan dengan empat primer ISSR PKBT dan hasilnya dipisahkan melalui gel agarose. Data dianalisis menggunakan program NTSYSpc versi 2:02. Hasil penelitian
menunjukkan bahwa amplifikasi DNA menggunakan empat primer ISSR BKPT menghasilkan 66 pita polimorfik. Hasil analisis menunjukkan bahwa keanekaragaman genetika durian lokal koleksi Hortimart tergolong tinggi karena 100% pita DNA polimorfik dengan variasi ukuran 250–1500 bp. Tidak ditemukan aksesi durian lokal yang memiliki profil DNA100% sama, meskipun empat pasang aksesi diketahui memiliki kemiripan tinggi 96%. Lima aksesi, yakni Petruk 2, Ontoseno, Semar, Arjuno, dan Gondomono memiliki pita DNA spesifik. Satu-satunya aksesi durian yang terpisah dari kelompok lainnya adalah Gondomono karena hanya memiliki satu pita pada ISSR PKBT 8 dengan ukuran 750 bp yang tidak dimiliki aksesi yang lain. Meskipun demikian, dendrogram memperlihatkan tidak ada identitas yang 100% sama sehingga setiap profil pita DNA dapat menjadi identitas
setiap aksesi durian.  


Kata kunci:  Durio zibethinus, penanda molekuler, keanekaragaman genetika.


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References


Alves MS, Nizio DAC, Brito FA, SampaioTS, Silva AVC, Arrigoni-Blank MF, Carvalho FVA & Blank AF. 2016. Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers. Genetic and Molecular Research 15 (4):1–10.

Arya L, Verma M & Lakhanpaul S. 2014. Diagnostic set of microsatellite markers for hybrid purity testing and molecular identification of hybrids and

parental lines in sorghum. Journal of Plant Science & Research 1(1):1–4.

Begum H, Reddy MT, Malathi S, Reddy BP, Narshimulu G, Nagaraju J & Siddiq EA. 2014. Morphological and microsatellite analysis of intravarietal heterogeneity in ‘Beneshan’ mango (Mangifera indica L.). International Journal of Agricultural and Food Research 3 (2):16–33.

Bumrungsri S, Sripaoraya E, Chongsiri T, Sridith K & Racey PA. 2009. The pollination ecology of durian (Durio zibethinus, Bombacaceae) in southern Thailand. Journal of Tropical Ecology 25: 85–92.

Feitosa-Alcantara RB, Silva AVC, Blank AF, Almeida CS, Alvares-Carvalho CV & Arrigoni-Blank MF. 2017. Analysis of genetic diversity of Hyptis pectinata (L.) Poit. plants using ISSR markers. Genetic Molecular Research

(3):1–10.

Geleta M & Bryngelsson T. 2009. Inter simple sequence repeat (ISSR) based analysis of genetic diversity of Lobelia rhynchopetalum (Campanulaceae). Hereditas 146:122–130.

Handayani F & Rahayu SP. 2017. Assessment of genetic diversity in lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker. Biodiversitas 18(2): 525–528.

Indriani FC, Sudjindro AN, Sugiharto & Soetopo L. 2008. Keragaman genetik plasma nutfah kenaf (Hibiscus cannabinus L.) dan beberapa

spesies yang sekerabat berdasarkan analisis isozim. Agritek 6(9): 1793–1802.

Kimman P. 2002. Biodiversity survey of the proposed Kelian protection Forest. PT. Kelian Equatiroal mining.

Kumar P, Gupta VK, Misra AK, Modi DR & Pandey BK. 2009. Potential of molecular markers in plant biotechnology. Plant Omics Journal Southern Cross Journal 2(4): 141–162.

Lim TK & Luders L. 2009. Boosting Durian Productivity. Darwin. Rural Industries Research and Development Corporation.

Mursyidin DH & Daryono BS. 2016. Genetic diversity of local durian (Durio zibethinus Murr.) Cultivars of South Kalimantan’s Province Based on RAPD Markers. Advances of Science and Technology for Society. AIP Conf. Proc. 1755:1–7.

Nei M & Li WH 1979. Mathematical model for studying genetic variation in terms of restriction endonucleasis. Proceedings of the National Academy of Sciences of the USA 76: 5269–5273.

Retnoningsih A, Megia R & Hartana A. 2010. Molecular verification and diversity analysis of Indonesian BB, AAB and ABB banana cultivars. Tree and Forestry Science and Biotechnology 4: 69–76.

Retnoningsih A. 2016. Kebun Benih Durian Unggul Indonesia: Menyediakan

Benih Unggul Bersertifikat untuk Pengembangan Kawasan Durian Unggul. Dalam: Rumah Ilmu Inovatif dan Membumi. Mastur Z, Cahyono E, Sudarmin & Kurniawan C. (eds.). FMIPA UNNES.

Retnoningsih A, Rahayu ES & Sari IP. 2017. Characterization of local durian germplasm based on the morphology of fruit. Jurnal Sains dan Teknologi 14(2): 89–94.

Rohlf FJ. 1998. NTSYSpc. Numerical Taxonomy and Multivariate Analysis System. User Guide. Departement of Ecology and Evolution. State University of New York. New York.

Saha K, Sinha RK, Basak S & Sinha S. 2016. ISSR Fingerprinting to Ascertain the Genetic Relationship of Curcuma sp. of Tripura. American

Journal of Plant Science’s 7: 259–266.

Semagn K, Bjornstad A & Ndjiondjop MN. 2006. An overview of molecular marker methods for plants. African Journal of Biotechnology 5(25): 2540–2568.

Stuessy TF, Takayama K, Sepulveda PL & Crawford DJ. 2014. Interpretation of patterns of genetic variation in endemic plant species of oceanic islands. Botanical Journal of the Linnean Society 174: 276–288.

Syahruddin K. 2012. Analisis keragaman beberapa genotipe durian (Durio zibethinus Murr.) menggunakan penanda morfologi dan molekuler (ISSR). Tesis. Bogor: Institut Pertanian Bogor.

Tirtawinata MR, Santoso PJ & Apriyanti LH. 2016. Durian: Pengetahuan Dasar untuk Pecinta Durian. Agro Flo.

Udupa SM & Baum M. 2001. High mutation rate and mutational bias at microsatellite in loci chickpea (Cicer arietium L.). Molecular Genet. Genomics 265: 1097–1103.

Uji T. 2005. Keanekaragaman jenis dan sumber plasma nutfah durian (Durio spp.) di Indonesia. Buletin Plasma Nutfah 11: 28–33.

Vanijajiva O. 2011. Genetic variability among durian (Durio zibethinus Murr.) cultivars in the Nonthaburi province, Thailand detected by RAPD analysis. Journal of Agricultural Technology 7(4): 1107–1116.

Vanijajiva O. 2012. The application of ISSR markers in genetic variance detection among durian (Durio zibethinus Murr.) cultivars in the Nonthaburi Province, Thailand. Procedia Engineering 32: 155–159.

Wang Y, Wang X & Paterson AH. 2012. Genome and gene duplications and gene expression divergence: a view from plants. Annals of The New York Academy of Sciences 1: 1–14.

Widiastuti A, Sobir & Suhartanto MR. 2013. Analisis keragaman genetik manggis (Garcinia mangostana) diradiasi dengan sinar gamma berdasarkan penanda ISSR. Bioteknologi 10(1): 15–22.

Yulianti F, Marasari C, Karsinah & Hartono T. 2010. Variasi genetik jeruk keprok SoE (Citrus reticulata Blanco) hasil radiasi sinar gamma menggunakan penanda ISSR. Buletin Plasma Nutfah 16(2): 134–139.

Yulita KS & Murnianjari. 2010. Keragaman genetic beberapa klon durian (Durio zibethinus Murray.) asal Jawa Barat berdasarkan sidik jari random

amplified polymorphic DNA. Berita Biologi 10(3): 269–275. Zulfahmi. 2013. Penanda DNA untuk analisis genetik tanaman. Jurnal Agroteknologi 3(2): 41–52.


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